| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Reasons, Kayla (7)
|
MAWAP |
48.94 |
48.23 |
-0.71 |
| 2 |
Fisher, Paige E (8)
|
MAGRA |
51.03 |
48.91 |
-2.12 |
| 3 |
Ying, Joy (8)
|
MAMSA |
1:05.93 |
49.98 |
-15.95 |
| 4 |
Rizzo, Isabella F (8)
|
MAGRA |
|
51.28 |
- |
| 5 |
Tallapragada, Harshitha T (8)
|
MAMSA |
52.17 |
52.27 |
0.10 |
| 6 |
Shultzabarger, Lexie B (8)
|
MAFSSC |
|
52.42 |
- |
| 7 |
Jefferson, McKinley F (8)
|
MAWAC |
53.12 |
52.79 |
-0.33 |
| 7 |
Shields, Hayden E (8)
|
MAWAP |
|
52.79 |
- |
| 9 |
Hahn, Milla G (8)
|
MAPAAC |
|
53.41 |
- |
| 10 |
Canavan, Lillian H (8)
|
MAGRA |
|
54.49 |
- |
| 11 |
Johnson, Eliza C (8)
|
MAWAC |
53.53 |
54.59 |
1.06 |
| 12 |
Wolbert, Sydney R (7)
|
MAPFY |
55.68 |
55.38 |
-0.30 |
| 13 |
Darmo, Gianna Marie (8)
|
MAGRA |
|
56.11 |
- |
| 14 |
Miller, Linnea R (8)
|
MAFSSC |
59.82 |
56.71 |
-3.11 |
| 15 |
Owens, Ava L (8)
|
MASSC |
|
57.50 |
- |
| 16 |
Harding, Kennedy O (8)
|
MAWAC |
54.27 |
58.45 |
4.18 |
| 17 |
McCullough, Kate T (8)
|
MABSC |
|
58.90 |
- |
| 18 |
Kovalenko, Katie M (7)
|
MAUPY |
|
1:03.52 |
- |
| 19 |
Isaacs, Mae (8)
|
MABSC |
1:05.38 |
1:03.59 |
-1.79 |
| 20 |
Jeffers, Lily A (7)
|
MAUPY |
|
1:08.30 |
- |
|
Whalen, Nora (8)
|
MABSC |
|
DQ |
- |
|
Hickey, Lillian G (7)
|
MASSC |
|
NS |
- |
|
Stetson, Hadley B (7)
|
MAEAAC |
|
NS |
- |